|Antinuclear Antibody in Putatively Healthy
|1Amina Hussein Hassab, 2Samir H. Asaad.
|1Departments Of Clinical Pathology and 2Internal
Medicine Faculty of Medicine, University of Alexandria, Egypt.
The purpose of this study was to determine the frequency of antinuclear
antibodies (ANA) in normal healthy Egyptians. Sera from apparently healthy
children (44) and adults (38) were tested for ANA by use of indirect immunofluoresence
using HEp-2 substrate. Sera from 25 patients (adults and children) diagnosed
to have an autoimmune disease were also tested. All sera were tested at
four different dilutions; 1:20, 1:40, 1:80, 1:160. Healthy individuals
had ANA at dilutions 1:20 & 1:40 (73.3% & 45.12%, respectively)
with sensitivity & negative predictive values of 100% at 1:20 serum
dilution. At serum dilution of 1:80, the sensitivity was 72%, specificity
100%, positive predictive value 100%, negative predictive value 92.1%.
It is recommended to use serum dilutions of 1:20 & 1:80 to screen and