1Amal I. Abd El-Hady, 1Elham A. El-Ebiary and 2Fekria A. El-Bourdiny
1Central Laboratory for Control of Veterinary Biologics and 2Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
In the present study, 2 inactivators (binary ethyleneimine
"BEI" and formalin) were compared for infectious bursal disease virus
(IBDV) inactivation. A recently described oil adjuvant (ISA-70) was used to
prepare a local BEI-inactivated IBD vaccine. This preparation was compared with
the imported vaccine preparation which has been inactivated by formalin and
adjuvanted by paraffin. Complete inactivation of 108 tissue culture
infective dose fifty (TCID50)/ml IBD virus occurred after 12 hours
by BEI and after 18 hours by formalin. Evaluation of the chick´s immune
response post vaccination was determined using enzyme linked immunosorbent
assay (ELISA), serum neutralization test (SNT) and quantitative agar gel
precipitation test (QAGPT). The locally prepared BEI-inactivated vaccine
induced higher antibody titres in the vaccinated chicks than the
formalin-inactivated vaccine. The highest antibody titres of antibodies were
obtained at 4th week post vaccination and continued to be protective
for the subsequent 12 weeks.