Elham A. El-Ebiary, Abdel-Hakam; MM., Susan S. El-Mahdy and Monier El-Safty
Central Laboratory for Control of Veterinary Biologics, Abbassia, Cairo, Egypt
A study was conducted on differentiation of infectious
laryngotracheitis (ILT) disease field isolates and vaccinal strain to
differentiate between the disease and post vaccinal reaction. Restriction
endonuclease (RE) digestion patterns of field isolates of ILT virus were
compared with two vaccinal strains. The field isolates generated RE digestion
pattern not identical to a vaccial strain by using EcoRI and BamHI enzymes,
which indicates that the field infection was due to field viruses not post
vaccinal reaction. RE provides additional tools for discrimination between ILT
strains.