T.C. ACT PLUS Immunopotentiated “CU” Live Fowl Cholera Vaccine.

AAR Khafagy.

Department of Microbiology, Faculty of Veterinary Medicine, Suez Canal University, Egypt.

A study was undertaken to investigate the possible implication of the nonspecific immunostimulant T.C. ACT PLUS,as a means of amplifying the immune response elicited by “C U”fowl cholera vaccine. In this study, vaccinated chickens treated with T.C. ACT PLUS developed a significant high (p<0.001) level of specific antibodies as measured by ELISA and IHA. On the other hand, T.C. ACT PLUS simultaneously administered with CU fowl cholera vaccine resulted in an enhancement of in vitro peripheral blood lymphocyte blastogenic response to PHA as compared with the control group. The lymphocyte immunostimulation indices however, showed significant differences (p<0.01) at 2 weeks post vaccination and at 1, 2, 3 and 4 weeks after boostering. Survival after challenge was 75% in chickens which were only vaccinated and 82.6% in chickens that were vaccinated and treated with T.C. ACT PLUS. Such information is quite sufficient to suggest that T.C. ACT PLUS has improved the immunopotentiating capacities of CU strain of live Pasteurella multocida vaccine. Protection against a virulant strain of Pasteurella multocida was evident when T.C. ACT PLUS treated unvaccinated chicken group was challenged where 39.28% survived, which was significantly higher than the 6.25% survival rate of untreated control. This shield of nonspecific protection could be established through cellular arm of immunity.