1SM Aboul Saoud, 1SM Gergis, 2FR El-Seedy, 1Nahed I Khamis and 3MA Abd El-Ghani.
1Department of Aerobic Bacterial Vaccines, Veterinary Serum and Vaccine Research Institute, Abbasia, 2Bacteriology, Mycology, & Immunology Department,, Beni Suef Branch and Department of 3Microbiology, Faculty of Veterinary Medicine, Cairo University.
In a trial to evaluate differently prepared S. enteritidis vaccines for controlling S. enteritidis infection and preventing its faecal shedding, the humoral immune response was measured by tube agglutination test, micro-agglutination test & ELISA, while cell mediated immunity was estimated by blastogenic response of peripheral blood lymphocyte to B cells mitogen (Pokeweed) and T cells mitogen (Concanavalin A). The bioassay was performed by challenging the immunity of vaccinated chicken by virulent strain of S. enteritidis. The outer membrane protein vaccine gave the best results as indicated by the highest tube agglutination, microagglutination, ELISA mean absorbency value, blastogenic response of peripheral B and T lymphocyte cells, highest protection value, and low percent of faecal shedding as well as lower reisolation rates after challenge. The acetone killed S. enteritidis oil adjuvant vaccine came next followed by the irradiated oil adjuvant vaccine. On the contrary, the formalized S. enteritidis oil adjuvant vaccine gave the lowest results. In conclusion the present study revealed the value of outer membrane protein vaccine for controlling S. enteritidis infection in chickens.