Khalifa E Khalifa, Maha M Abou Gamra, [†]Sahar S Abdel-Aziz and Laila M El-Hoseiny.
Department of
Parasitology, Faculty of Medicine, Ain Shams University.
The BCG adjuvant effect on vaccination against Trichinella spiralis was evaluated in two slow responders: outbred Swiss albino and inbred C57BL/10 strains of mice. The mice were vaccinated i.d. with BCG and /or excretory-secretory (ES) antigen before oral challenge with 300 infective T. spiralis larvae. BCG/ES vaccination of the outbred Swiss albino mice resulted in highly significant reduction of worm recovery on day 12 (P< 0.001) and significant reduction of female fecundity and muscle larvae on days 7 and 35, respectively (p<0.05). Separate vaccination with either BCG or ES resulted in non significant changes of the parameters mentioned above. With the C57BL/10 mice, the BCG/ES vaccination resulted in a significant reduction of all the above mentioned parameters. However, worm recovery on day 12 from BCG/ES vaccinated Swiss albino mice was significantly lower than from BCG/ES vaccinated C57BL/10 mice. The outbred Swiss albino mice vaccinated with BCG/ES, BCG and ES/ES mounted an earlier anti-Trichinella specific IgG antibody response than that displayed by infected unvaccinated mice. While in inbred C57BL/10, only BCG/ES vaccination resulted in earlier appearance of antibodies. In comparison with the control group, in which the antibody response was peaked on day 35, all the vaccinated groups of both strains except BCG vaccinated C57BL/10 showed peak antibody response on day 21.
[†] Correspondence: Dr. Sahar Safwat Abdel Aziz;
Parasitology Department. Faculty of Medicine, Ain Shams University, Abassia
Cairo, Egypt.