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ICAM-1 and LFA-1 Expression Increased in the Skin After Vaccination with Ultraviolet Attenuated Schistosoma of Mice.
1Soad E. Abdel Rehim, 1El-Gendy A.M., 2Mohamed Kasem and 1El-Sheikh N.
1Microbiology department, Faculty of Medicine for Girls and 2Histology department, Faculty of Medicine (Assiut Branch), Al-Azhar University.

Previous studies have shown that the differential expression of cell-adhesion molecules plays a critical role in cutaneous inflammation and immunologic responses. Intercellular adhesion molecule-1 (ICAM-1) is constitutively expressed on endothelial cells lining dermal capillaries and keratinocytes and serves as specific ligand for lymphocyte function associated antigen-1 (LFA-1). The present study describes the expression of ICAM-1 and its cognate integrin LFA-1 during skin phase immunity after vaccination and challenge infection of Schistosoma mansoni. An ultraviolet (U.V.) attenuated vaccine model was used, C57Bl/6 mice were vaccinated by tail immersion and a piece of the tail was cut from naive (N), vaccinated (V), vaccinated/challenged (VC) and naive/challenged (NC) mice groups at day 1, 4 and 6 after exposure. Tail cryostat sections were stained with FITC-monoclonal antibodies (mAb) to ICAM-1 and LFA-1 and examined semi-quantitatively by direct immunoflourescence. Expression of ICAM-1/LFA-1 was markedly enhanced in the (V) and (VC) as compared with that of (N) and (NC) mice groups respectively. kinetic analysis reveled that expression was high at day 1,4 and 6 for ICAM-1, while that for LFA-1 was only at day 1. Thus, an ICAM-1/LFA-1 mediated early accumulation of mononuclear cells in the skin may be important for the initial cutaneous inflammatory immune responses to migrating schistosomula of S. mansoni in vaccinated animals. In the contrary, in naive animals a potential parasite-induced suppression of adhesion molecules expression in the skin may consequently help the parasite evade immune response in the skin. The enhanced expression of ICAM-1/LFA-1 correlates and coincide with the massive mononuclear cell infiltration as demonstrated by H&E.

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