|ICAM-1 and LFA-1 Expression Increased in the
Skin After Vaccination with Ultraviolet Attenuated Schistosoma of Mice.
|1Soad E. Abdel Rehim, 1El-Gendy A.M., 2Mohamed
Kasem and 1El-Sheikh N.
|1Microbiology department, Faculty of Medicine for Girls
and 2Histology department, Faculty of Medicine (Assiut Branch),
Previous studies have shown that the differential expression of cell-adhesion
molecules plays a critical role in cutaneous inflammation and immunologic
responses. Intercellular adhesion molecule-1 (ICAM-1) is constitutively
expressed on endothelial cells lining dermal capillaries and keratinocytes
and serves as specific ligand for lymphocyte function associated antigen-1
(LFA-1). The present study describes the expression of ICAM-1 and its cognate
integrin LFA-1 during skin phase immunity after vaccination and challenge
infection of Schistosoma mansoni. An ultraviolet (U.V.) attenuated vaccine
model was used, C57Bl/6 mice were vaccinated by tail immersion and a piece
of the tail was cut from naive (N), vaccinated (V), vaccinated/challenged
(VC) and naive/challenged (NC) mice groups at day 1, 4 and 6 after exposure.
Tail cryostat sections were stained with FITC-monoclonal antibodies (mAb)
to ICAM-1 and LFA-1 and examined semi-quantitatively by direct immunoflourescence.
Expression of ICAM-1/LFA-1 was markedly enhanced in the (V) and (VC) as
compared with that of (N) and (NC) mice groups respectively. kinetic analysis
reveled that expression was high at day 1,4 and 6 for ICAM-1, while that
for LFA-1 was only at day 1. Thus, an ICAM-1/LFA-1 mediated early accumulation
of mononuclear cells in the skin may be important for the initial cutaneous
inflammatory immune responses to migrating schistosomula of S. mansoni
in vaccinated animals. In the contrary, in naive animals a potential parasite-induced
suppression of adhesion molecules expression in the skin may consequently
help the parasite evade immune response in the skin. The enhanced expression
of ICAM-1/LFA-1 correlates and coincide with the massive mononuclear cell
infiltration as demonstrated by H&E.