|Assessment of Liver Granuloma in Schistosoma
mansoni Infected and Vaccinated Mice using Cell Image Analyzer.
|1Nassar MI, 2Sayed Al-Ahl S, 3Abdel
Ghaffar AB and 4El-Sheikh N.
|1Pathology department, Assiut University, 2Parasitology
Department and 4Microbiology Department, Al-Azhar University
and 3Biochemistry Department, Faculty of Science, Ain Shams
University, Cairo, Egypt.
The UV-irradiated S.mansoni cercariae vaccine model was used to investigate
the effect of vaccination on morbidity, fertility and fecundity. The changes
in granuloma size, as an indicator of change in morbidity, was assessed.
Bilharzial liver granulomas from Balb /c vaccinated/challenged (V/C) and
naïve/challenged (NC) mice were compared. Serial and multiple sections
from different lobes of the liver were examined at eight weeks post challenge
infection. The morbidity studies included analysis of the ratio between
granuloma tissue/section to total tissue area /section, this was done by
topographic morphometry which allows the measurement of a large specimen
across multiple fields of view in a single pass using image analyzer. In
addition to the number of granuloma, the net granuloma area, diameter,
and the longest granuloma dimension were assessed. Results in V/C mice
group showed a reduction in the mature worm burden by 62 %, an increase
the male to female ratio by 1 to 3 and a reduction in the fertility and
fecundity of the worm to 45%. A 30% reduction in the no. of ova/ gram intestinal
tissue and stools was observed, however, there was no change in the mean
no. of retained ova/ gram liver tissue. Morphological results showed no
significant interlobular variations. The mean net granuloma surface area
values in microns were 15403.57±12749.32, and 32099.57±21936.
and the ova area was 1249.69±852.03 and 1596.41±929.71 for
V/C and NC mice groups respectively. No correlation was found between the
reduction in the granuloma size of vaccinated animals and the worm burden,
however, it was correlated with the decreased number of viable ova production
by adult S.mansoni worms inside the vaccinated group. This reduction reduced
the morbidity by 47%. It is concluded that extended mode analysis by image
analyzer enforce the measurement of granuloma area and viability of passed
ova as an indicator for change in morbidity.