|Tumor Associated Glycoprotein in Patients
with Schistosomal Urinary Bladder Cancer Establishment of A Diagnostic
ELISA Technique Using Polyclonal Antibody.
|El-Asfahani, A.M.A.1, Shaheen, A.M.1, Kamal, K.A.2,
Shaheen, H.2, and Attallah, A.M.2
|1Biochemistry Department, Faculty of Medicine, Al-Azhar
University, Cairo, Egypt and 2Immunology Department, NAMRU-3,
Polyclonal rabbit antiserum was prepared against a tumor associated
glycoprotein or antigen (TAA) isolated by Con-A immunoaffinity from urine
of patients with schistosomal urinary bladder carcioma (SUBC) of squamous
cell carcinoma (SCC) type. An absorbed rabbit antiserum was prepared by
repeated immunoadsorption techniques using normal urine and human serum.
An enzyme linked immunosorbent assay (ELISA) was established for detection
of urinary TAA using the absorbed polyclonal rabbit antiserum. The reactivity
of this antiserum was almost eliminated against normal urine but it was
reactive against SUBC (83%), schistosomal cystitis (75%), and other cancers
(13.3%). The sensitivity and specificity of the ELISA were 83% and 74%,
respectively while the overall accuracy was 77%. This study shows that
the urine from patients with SUBC might have a Con-A binding glycoprotein,
or TAA which partially cross reacted with a similar urinary glycoprotein
from patients with schistosomal cystitis. It could be suggested that this
absorbed polyclonal antibody might be used for further isolation of tumor
associated glycoprotein antigens from urine of SUBC patients for production
of monoclonal antibodies specific for this cancer.