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Tumor Associated Glycoprotein in Patients with Schistosomal Urinary Bladder Cancer Establishment of A Diagnostic ELISA Technique Using Polyclonal Antibody.
El-Asfahani, A.M.A.1, Shaheen, A.M.1, Kamal, K.A.2, Shaheen, H.2, and Attallah, A.M.2
1Biochemistry Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt and 2Immunology Department, NAMRU-3, Cairo, Egypt.

Polyclonal rabbit antiserum was prepared against a tumor associated glycoprotein or antigen (TAA) isolated by Con-A immunoaffinity from urine of patients with schistosomal urinary bladder carcioma (SUBC) of squamous cell carcinoma (SCC) type. An absorbed rabbit antiserum was prepared by repeated immunoadsorption techniques using normal urine and human serum. An enzyme linked immunosorbent assay (ELISA) was established for detection of urinary TAA using the absorbed polyclonal rabbit antiserum. The reactivity of this antiserum was almost eliminated against normal urine but it was reactive against SUBC (83%), schistosomal cystitis (75%), and other cancers (13.3%). The sensitivity and specificity of the ELISA were 83% and 74%, respectively while the overall accuracy was 77%. This study shows that the urine from patients with SUBC might have a Con-A binding glycoprotein, or TAA which partially cross reacted with a similar urinary glycoprotein from patients with schistosomal cystitis. It could be suggested that this absorbed polyclonal antibody might be used for further isolation of tumor associated glycoprotein antigens from urine of SUBC patients for production of monoclonal antibodies specific for this cancer.

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Copyright 2002 Egyptian Association of Immunologists .
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