1Eman I. Kandil, 1Samir F. Zohny, 1Said S. Moselhy, 2Ehab M. Mohamed and 3Magdy M.Khalil.
1Biochemistry Department, Faculty of Science, Ain Shams University, 2Chemistry Department, Faculty of Science, Tanta University, 3Chest Department, Faculty of Medecine, Ain Shams University.
The mite allergens are recognized as
major causes of allergic diseases such as bronchial asthma, allergic rhinitis
and atopic dermatitis. In an attempt to identify the different mite antigen
that bind human specific IgG, a group of patients with Dermatophagoides allergy
was studied. Also, the behaviour of blocking antibodies directed against
complete allergen extract Dermatophagoides pteronyssinus (D.pt) and Dermatophagoides
farinae (D.f) was analyzed using immunoblot analysis. These results showed
binding of specific IgG antibodies to component of D.f and D.pt antigen with M.w range 25-94 kDa for D.f and 14-140
kDa for D.pt. Also, binding of specific IgG4 antibodies to components of D.f
antigen was identified at M.W. 60 and 67 kDa. These results confirm that
antigenically distinct allergens exist. In conclusion, the SDS-PAGE and
immunoblot analysis clearly demonstrate that the presence of allergenically
important major proteins was readily proved, and that they were highly suitable
for screening specificity of antibodies in patient sera.