Ayad Samia, Mouaz Mohamed, Kamel Nahed, Abdel-Wahab Afaf and Daoud Ahmed.
Rinderpest Research and
Vaccine Production Department, Veterinary Serum and Vaccine Research Institute,
Abbasia, Cairo, Egypt.
Two peste des petits ruminants virus (PPRV) vaccine batches
(A and B) were manufactured synchronously with an attenuated PPRV on Vero
cells. Aspects of vaccine batch A were: a) incorporation of 1% monosodium
glutamate (MSG) in the PPRV inoculum for 5 roller Vero cell culture bottles, b)
incorporation of 1% of the same salt in the vaccine stabilizer (wt/volume) and
c) incorporation of 1% of the same salt in the vaccine diluent (normal
physiological saline solution). These aspects were lacking in PPRV vaccine
batch B. Comparative studies revealed that, the incorporation of MSG resulted
in a) detectable enhancement of PPRV yields in roller culture bottles of Vero
cells, b) yield antibody stability in vaccinated susceptible sheep and c)
significant improvement of thermostability potential of the lyophilized vaccine
at -20oC. The actual mechanism of action of MSG in these respects
has to be assessed.