Make your own free website on

Influence of Fungal Chemotactic Activity on Mononuclear and Polymorphonuclear Leukocytes.

1MM El-Shikh, 1AA Abou-Seada, 2MA Abdel-Nasser and 2MM Sherif

1Regional Centre for Mycology & Biotechnology; 2Department of Microbiology & Immunology, Faculty of Medicine; Al-Azhar University.

Fungal chemotaxis, as a cornerstone phenomenon in cell-mediated immunity, received little or no attention. The current study aimed at investigating the chemotactic activity of Aspergillus niger, Alternaria alternata and Syncephalastrum racemosum towards mononuclear and polymorphonuclear (PMN) leukocytes. The leukotactic index (LI) was measured and expressed in mm (Boyden technique) as well as the ratio of induced migraion/ spontaneous migration (Nelson technique). Different concentrations of fungal mycelia with or without normal serum were used in paralell to negative and positive chemoattractants. The study revealed an inhibitory activity for the different concentrations of A niger and A alternata  (LI <1 by Nelson technique and 26.8-38.8 mm by Boyden technique, while S racemosum exhibited a stimulatory effect (LI >1 by Nelson technique and 35.7-40.1 mm by Boyden technique). Except for monocyte’s responses to S racemosum in Boyden technique (p=0.01), no significant difference was found between the responses of PMNs and that of monocytes or lymphocytes towards any of the fungal isolates (P>0.5). Addition of serum, displayed more or less insignificant role in mediating fungal chemotaxis. The results recorded via the two different chemotaxis assays were almost identical confirming their validity.