A.F. Wahby.
Department of Molecular
Biology, National Research Center, Dokki, Cairo, Egypt.
A new assay, the Cell-Associated Virus ELISA (CAV-ELISA) for
detection of antiviral antibodies was established. The assay is based on using
cell-associated virus antigens (CAV) as an alternative for the
virus-senthetized ELISA plates. Smooth dose response curves were obtained for
the binding of rabbit antipoliovirus antibodies with BGM associated poliovirus.
At relatively high concentrations of CAV, superimposed dose response curves
were reproducible over a wide range of virus concentrations, and binding become
a function of serum concentration. At relatively lower concentrations of CAV
the bound antibodies decreases with both the virus and antibody concentrations.
Standard dose response curves expressing the binding of different antibody
concentrations to the CAV were established and used for determination of the
levels of antibodies in test samples. The interpreted titers from standard dose
response curves were highly reproducible. Furthermore, the CAV-ELISA is as
sensitive as the indirect ELISA and is minimally affected by the variations in
virus preparation.