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Evaluation of Humoral and Cellular Immune Responses to African Horse Sickness (AHS) Inactivated Vaccine Adjuvanted with Either Oil or Rehydragel.

El-Kabbany MMA, Soliman IM, Magda A Kalad and Madkour MS.

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo.

The present study was adopted to evaluate the humoral and cellular immune responses in horses vaccinated with the inactivated vaccine adjuvanated with either oil or rehydragel. The African Horse Sickness (AHS) virus serotype 9 was inactivated at 37oC by addition of binary ethyleneimine (BEI) at a final concentration of 0.004 M for 24 hours. Seven adult susceptible horses were divided into 2 groups (3 horses/group) and one acted as control. The first group (A) was inoculated intramuscularly with inactivated AHS/Oil vaccine and the 2nd group (B) with inactivated AHS rehydragel vaccine. The humoral immune response was evaluated using serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA), while the cellular immune response was evaluated using lymphocyte transformation test. Horse in group B produced earlier activity of cell mediated immune response than those vaccinated with inactivated AHS oil vaccine (group A). In contrast (group A) demonstrated earlier humoral immune response than (group B).