1Farha A. El-Chennawy 2Samia E. Abd El-Naby, 1Fatma A. Auf, 1Shirin S. Metwally and 1Elham R.Abd EL -Sameea
1Immunology Unit, Clinical Pathology Department, 2Dermatology and Venereology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
To determine the association
of HLA molecules with systemic lupus erythematosus (SLE) and the clinical
findings of the disease, HLA antigens were tested in 21 Egyptian patients with
SLE who fulfilled the American Rheumatism Associations (ARA) diagnostic
criteria. HLA class I was typed serologically using lymphocyte
microcytotoxicity test, and HLA class II DRB1 and DQB1 alleles were defined by
using the PCR/SSOP method. Anti-double stranded DNA (ds-DNA) antibodies were
detected by enzyme-linked immunosorbent assay (ELISA). Among the Egyptian
patients, the typing results show increased frequency of HLA class I A3, A25,
B13, B35 antigens and HLA class II DRB1 *0301 allele in patients with SLE
compared to normal controls. There are some positive associations between HLA
antigens and clinical findings in SLE such as malar rash with A1, B13 antigens
and DQB1 *0401 allele, alopecia with B5, DRB1 *1501, DQB1 *0401,
photosensitivity with A2, B5, B35, DRB1 *0602 and arthritis with A3, B35, DRB1
*0301, and DQB1 *0602. We conclude that there are some associations of HLA
markers with SLE and its clinical manifestations.