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Increased mRNA Levels of Eotaxine and Monocyte Chemotactic Protein-3 (MCP-3) in Bronchoalveolar Lavage Fluid of Asthmatic Patients

1Maha Salah El-Din Hamdy, 2Laila Ashour Mohamed, 2May El-Attar, 3Sahar Samir Abdel-Maksoud and 4Mohamed Attya Zamzam

departments of 1Microbiology and immunology, 2Chest, 3Clinical pathology, school of medicine, Ain Shams University and 4Chest department Menofya University, Egypt.

Chemokines are a group of cytokines that induce chemotaxis of the inflammatory cells, mediating the pathophysiology of asthma. This work investigates production of chemokines namely eotaxine and monocyte chemotactic protein-3 (MCP-3) in bronchoalveolar lavage fluid (BALF), obtained from 24 asthmatic patients (8 mild and 16 moderate) who met the Egyptian guidelines for diagnosis and management of asthma. 8 non-asthmatic patients who needed diagnostic bronchoscopy were included as controls. The cells from BALF were pelleted, and ribonucleic acid (RNA) was extracted and amplified using a semiquantitative polymerase chain reaction (PCR). The results revealed a significant increase in the (mRNA) level for MCP-3 and eotaxine in asthmatics as compared to that of controls (P<0.001). The mRNA levels were 0.28 0.08, 2.19 0.51 & 1.99 0.7 pg/ml for MCP-3 and 0.29 0.10, 1.67 0.66 & 1.98 0.54 pg/ml for eotaxine among the controls, mild and moderate cases of asthma respectively. The differences between mild and moderate cases were non-significant (p>0.05) for both MCP-3 and eotaxine in BALF. The eotaxine mRNA level correlated with the eosinophilic count in the BALF of the moderate asthmatics patients (r=0.550 and p<0.05). It is concluded that asthma is associated with combined expression of eotaxine and MCP-3, and that the degree of expression correlates positively with the eosinophil count and negatively with the response to usual lines of treatment. These findings could be considered as a promising therapeutic approach by blockade of the chemokine receptors.