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Differences In Th1/Th2 Cytokine Profiles in Ulcerative Colitis And Crohn’s Disease.

1Nahed Baddour, 2Mohamed Tamer Afifi.

Departments of 1Pathology and 2Internal medicine, Faculty of Medicine, Alexandria University.

To highlight the differences in the patterns of interleukin-10 (IL-10) and interferon gamma (IFN-g) cytokine expression between ulcerative colitis (UC) and Crohn's disease (CD), 45 endoscopic colonic biopsies were studied. They included biopsies from 10 cases of UC, 5 biopsies from active sites of CD, 5 from inactive sites of CD, 15 cases of noninflammatory bowel disease (NIBD) and 10 controls. All biopsies were subjected to immunohistochemical staining using monoclonal anti-IL-10 antibody and polyclonal anti-IFN g antibody. Quantitative analysis of positive signals were performed using a point counting technique. IL-10 was expressed in the intestinal epithelial cells, and in the mononuclears of the lamina propria. Higher levels of expression were detected in UC (1.44±1.28) than active and inactive CD lesions and NIBD and controls 0.67±0.66, 0.59±0.67, 0.69±0.84, 0.67±0.48 repectively (p<0.05). Expression of IFN-g was significantly higher in both active and inactive sites of CD (2.19±0.87, 0.66±0.99 respectively) than UC, NIBD and controls (0.24±0.54, 0.60±0.84, and 0.62±1.07 respectively)(p=< 0.05). It is concluded that there is a predominantly Th1 type response in cases of CD and Th2 type response in UC.