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Significance of Platelets Derived Growth Factor–AB and Nitric Oxide In Newborns Suffering From Perinatal Asphyxia.

Naima Kh. Aly, Sahar S. khatab and Safaa El-Meneza.

Departments of Clinical Pathology and Pediatrics Faculty of medicine for girls, Al Azhar University.

Platelet derived growth factor (PDGF) is secreted from the α -granules of platelets and is known to be a major mitogen for connective tissue cells including, dermal fibroblasts, gliat cells, and some epithelial cells. PDGFs are an important neurotropic peptides which have been found to be increased during perinatal asphyxia (PA). Nitric oxide (NO) is a major secretory product of mammalian cells, that has several biological activities in immune, vascular, neurological and other systems. Elevated NO level after perinatal asphyxia was found to be related to severity of brain damage and neurological outcome. PDGF- AB and NO were measured in sixty-four infants (using ELISA and colorimetric techniques respectively) to study their relation to perinatal asphyxia (PA). Twelve apparently healthy newborns were chosen as a control group. Newborns suffering from PA were further subdivided into those with ischaemic heart (26 cases) and with neurological manifestations (26 cases). There was a highly significant increase in PDGF-AB in asphyxiated newborns as compared to controls (P<0.001). The mean value of PDGF-AB in PA with neurological manifestations (116.13 ± 111.12 pg/ml) was higher than that in PA with ischaemic heart (85.85 ± 59. 05 pg/ml). Similarly NO results showed significant increase in PA newborns as compared to control (P<0.001), and the mean value was higher in patients with ischaemic heart (130.64 ± 34.59 μ mol/ ml), than with neurological manifestations (127.38 ± 33.96 μ mol/ ml.). In conclusion, PDGF- AB may play a protective role in PA with ischaemic heart and neurological manifestations while NO may be protective only in PA with ischaemic heart.