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Evaluation of Relationships Among ELISA, Dot ELISA and Agar Gel Precipitation Tests in The Detection of 3 CD Antigens of FMDV

M Shawky H El-Watany, A Samira El-Kilany and OH.Roshdy

FMD Research Department, Veterinary Serum & Vaccine Research Institute, Abbasia, Cairo.


Dot ELISA was used for the detection of antibodies against protease and polymerase genes (3 CD) antigens in animals infected with foot and mouth disease virus (FMDV). Antigen antibody reactions were detected with an antibovine serum labeled with horseradish peroxidase. Using 4-chloro-1-naphthol as a substrate, positive reactions appeared as blue dots and the results were compared with those of solid phase ELISA and agar gel precipitation. Dot ELISA appeared to have a potential for rapid and economical field test in the diagnosis of FMD in infected animals.