M Shawky H El-Watany, A Samira El-Kilany and OH.Roshdy
FMD Research Department, Veterinary Serum & Vaccine
Research Institute, Abbasia, Cairo.
Dot ELISA was used for the detection of antibodies
against protease and polymerase genes (3 CD) antigens in animals infected with
foot and mouth disease virus (FMDV). Antigen antibody reactions were detected
with an antibovine serum labeled with horseradish peroxidase. Using
4-chloro-1-naphthol as a substrate, positive reactions appeared as blue dots
and the results were compared with those of solid phase ELISA and agar gel
precipitation. Dot ELISA appeared to have a potential for rapid and economical
field test in the diagnosis of FMD in infected animals.